Date published: 2025-9-9

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Blocking Reagents

Santa Cruz Biotechnology now offers a broad range of blocking reagents for use in various applications. Blocking reagents are essential tools in biochemical and molecular biology experiments, particularly in techniques such as Western blotting, ELISA, and immunohistochemistry. These reagents work by occupying nonspecific binding sites on membranes or other surfaces, thereby preventing non-specific binding of antibodies or other detection molecules. This process enhances the specificity and clarity of experimental results, allowing researchers to obtain accurate and reliable data. In scientific research, blocking reagents are crucial for minimizing background noise and improving the signal-to-noise ratio in assays, which is vital for the detection and quantification of target molecules. They are also employed in the preparation of samples and surfaces in various types of assays and analytical procedures. By providing high-quality blocking reagents, Santa Cruz Biotechnology supports a wide range of scientific disciplines, including cell biology, biochemistry, and molecular genetics. These reagents enable researchers to conduct experiments with greater precision and reproducibility, facilitating advancements in our understanding of biological processes and the development of new technologies. View detailed information on our available blocking reagents by clicking on the product name.
Product NameCAS #Catalog #QUANTITYPriceCitationsRATING

UltraCruz® Blocking Reagent

sc-516214
100 ml
$77.00
59
(3)

UltraCruz® Blocking Reagent is designed to minimize non-specific binding in assays, enhancing the specificity of detection methods. Its unique formulation interacts with various proteins and surfaces, effectively saturating potential binding sites. This reduces background noise and improves signal clarity, allowing for more accurate results. The reagent's optimized composition ensures compatibility with a wide range of experimental conditions, making it a versatile tool in research applications.

Blotto, non-fat dry milk

sc-2324
sc-2325A
sc-2325
250 g
5 kg
500 g
$36.00
$562.00
$65.00
195
(14)

Blotto, non-fat dry milk, serves as an effective blocking agent through its unique protein composition, which interacts with various nucleophiles. The presence of lactose and whey proteins contributes to its ability to form non-covalent interactions, such as hydrogen bonding and hydrophobic effects. These interactions can modulate reaction pathways, influencing selectivity and stability in complex mixtures. Its physical properties, including solubility and viscosity, further enhance its blocking capabilities in diverse chemical environments.

TBS Blotto A

sc-2333
makes 500 ml
$26.00
51
(0)

TBS Blotto A is a versatile blocking agent that exhibits strong reactivity due to its unique structure, allowing it to form stable covalent bonds with nucleophiles. Its ability to selectively modify functional groups enhances its utility in various chemical pathways. The compound's steric hindrance and electronic properties facilitate specific interactions, leading to efficient blocking in complex reaction environments. This specificity can significantly influence reaction kinetics and product formation.

Boc-Met-Leu-Phe-OH

67247-12-5sc-293900
sc-293900A
100 mg
250 mg
$140.00
$340.00
(0)

Boc-Met-Leu-Phe-OH acts as a blocking agent by leveraging its specific amino acid sequence, which facilitates selective interactions with reactive sites. The Boc (tert-butyloxycarbonyl) group provides steric hindrance, preventing unwanted reactions while maintaining stability. Its hydrophobic side chains enhance molecular interactions through van der Waals forces, influencing reaction kinetics. Additionally, the compound's solubility characteristics allow for effective integration into various chemical systems, optimizing performance in diverse applications.

TBS Blotto B

sc-2335
makes 500 ml
$26.00
9
(2)

TBS Blotto B functions as a blocking agent through its unique structural features that promote selective binding to reactive sites. The presence of the TBS (tert-butyldimethylsilyl) group imparts significant steric protection, effectively shielding functional groups from undesired interactions. This compound exhibits enhanced stability and solubility, facilitating its integration into various chemical environments. Its ability to modulate reaction pathways and kinetics makes it a versatile choice for controlling reactivity in synthetic processes.

Tween-20

9005-64-5sc-29113C
sc-29113
sc-29113B
sc-29113A
100 ml
500 ml
1 L
3.8 L
$18.00
$30.00
$50.00
$160.00
54
(5)

Tween-20 acts as a blocking agent by leveraging its hydrophilic and hydrophobic regions, which facilitate effective adsorption onto surfaces. This amphiphilic nature allows it to form a stable layer that minimizes non-specific binding, enhancing the specificity of subsequent interactions. Its unique molecular structure promotes favorable van der Waals forces and hydrogen bonding, contributing to its effectiveness in preventing unwanted reactions in diverse biochemical assays.