ACCα Antibody (D-5): sc-137104

ACCα Antibody (D-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects ACCα protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ACCα (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Acetyl-CoA carboxylase (ACC) plays a crucial role in lipid metabolism, catalyzing the carboxylation of acetyl-CoA to malonyl-CoA, which is the rate-limiting step in fatty acid synthesis. This enzymatic activity regulates the production of long-chain fatty acids, essential for energy storage and membrane synthesis. ACCα isoform, also known as ACC1, is predominantly expressed in lipogenic tissues and regulated by phosphorylation and dephosphorylation of specific serine residues, as well as by allosteric interactions with metabolites such as citrate and palmitoyl-CoA. These regulatory mechanisms ensure fatty acid synthesis is appropriately modulated in response to cellular energy demands and nutritional status. Located on human chromosome 17, ACCα plays a significant role in metabolic pathways. Understanding ACCα function and regulation is vital for elucidating its role in metabolic disorders, including obesity and diabetes, making anti-ACCα antibody (D-5) an essential tool for researchers studying lipid metabolism and related diseases.

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ACCα Antibody (D-5) References:

1. The subcellular localization of acetyl-CoA carboxylase 2.  |  Abu-Elheiga, L., et al. 2000. Proc Natl Acad Sci U S A. 97: 1444-9. PMID: 10677481
2. Exercise diminishes the activity of acetyl-CoA carboxylase in human muscle.  |  Dean, D., et al. 2000. Diabetes. 49: 1295-300. PMID: 10923628
3. Cloning of human acetyl-CoA carboxylase beta promoter and its regulation by muscle regulatory factors.  |  Lee, JJ., et al. 2001. J Biol Chem. 276: 2576-85. PMID: 11076940
4. Activation of acetyl-CoA carboxylase by a glutamate- and magnesium-sensitive protein phosphatase in the islet beta-cell.  |  Kowluru, A., et al. 2001. Diabetes. 50: 1580-7. PMID: 11423479
5. Acetyl-CoA carboxylase alpha gene and breast cancer susceptibility.  |  Sinilnikova, OM., et al. 2004. Carcinogenesis. 25: 2417-24. PMID: 15333468
6. RNA interference-mediated silencing of the acetyl-CoA-carboxylase-alpha gene induces growth inhibition and apoptosis of prostate cancer cells.  |  Brusselmans, K., et al. 2005. Cancer Res. 65: 6719-25. PMID: 16061653
7. Acetyl-CoA carboxylase alpha is essential to breast cancer cell survival.  |  Chajès, V., et al. 2006. Cancer Res. 66: 5287-94. PMID: 16707454
8. Acetyl-CoA carboxylase-alpha inhibitor TOFA induces human cancer cell apoptosis.  |  Wang, C., et al. 2009. Biochem Biophys Res Commun. 385: 302-6. PMID: 19450551
9. Involvement of ACACA (acetyl-CoA carboxylase α) in the lung pre-metastatic niche formation in breast cancer by senescence phenotypic conversion in fibroblasts.  |  Huang, YC., et al. 2023. Cell Oncol (Dordr). 46: 643-660. PMID: 36607556
10. Identification of acetyl-CoA carboxylase alpha as a prognostic and targeted candidate for hepatocellular carcinoma.  |  Shen, Y., et al. 2023. Clin Transl Oncol. 25: 2499-2513. PMID: 36976490
11. Nicotinate-curcumin improves NASH by inhibiting the AKR1B10/ACCα-mediated triglyceride synthesis.  |  Lin, XL., et al. 2024. Lipids Health Dis. 23: 201. PMID: 38937844
12. Regulation of mammalian acetyl-coenzyme A carboxylase.  |  Kim, KH. 1997. Annu Rev Nutr. 17: 77-99. PMID: 9240920

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACCα Antibody (D-5) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-ACCα Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACCα Antibody (D-5) Specifications

Alternate Product Name: Acetyl-CoA Carboxylase Alpha

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-76

Epitope Target: ACCα

Epitope Species: human

Product Reactivity/Detection: ACCα

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for ACCα Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-ACCα antibody is p-ACCα Antibody (F-2): sc-271965.