Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (H-4): sc-374076

ALDH1A1 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ALDH1A1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ALDH1A1 (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ALDH1A1, also known as retinal dehydrogenase 1, plays a crucial role in retinoic acid biosynthesis, a vital signaling molecule involved in embryonic development, cell differentiation, and immune function. ALDH1A1 catalyzes retinaldehyde conversion to retinoic acid, essential for regulating gene expression and maintaining proper cellular functions. ALDH1A1′s importance extends to aldehydes detoxification, particularly in alcohol-derived acetaldehyde, corticosteroids, and neurotransmitter metabolism, highlighting significance in metabolic pathways and potential therapeutic targets in cancer treatment. ALDH1A1 upregulation in certain tumors suggests involvement in oxidative stress response and chemotherapy agent metabolism, making ALDH1A1 a valuable marker for cancer research and diagnostics.

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Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (H-4) References:

1. Molecular analysis of two closely related mouse aldehyde dehydrogenase genes: identification of a role for Aldh1, but not Aldh-pb, in the biosynthesis of retinoic acid.  |  Hsu, LC., et al. 1999. Biochem J. 339 (Pt 2): 387-95. PMID: 10191271
2. Eukaryotic aldehyde dehydrogenase (ALDH) genes: human polymorphisms, and recommended nomenclature based on divergent evolution and chromosomal mapping.  |  Vasiliou, V., et al. 1999. Pharmacogenetics. 9: 421-34. PMID: 10780262
3. Aldehyde dehydrogenase (ALDH) 2 associates with oxidation of methoxyacetaldehyde; in vitro analysis with liver subcellular fraction derived from human and Aldh2 gene targeting mouse.  |  Kitagawa, K., et al. 2000. FEBS Lett. 476: 306-11. PMID: 10913633
4. cDNA cloning and expression of a human aldehyde dehydrogenase (ALDH) active with 9-cis-retinal and identification of a rat ortholog, ALDH12.  |  Lin, M. and Napoli, JL. 2000. J Biol Chem. 275: 40106-12. PMID: 11007799
5. Negative regulation of the murine cytosolic aldehyde dehydrogenase-3 (Aldh-3c) gene by functional CYP1A1 and CYP1A2 proteins.  |  Vasiliou, V., et al. 1992. Biochem Biophys Res Commun. 187: 413-9. PMID: 1520328
6. Analysis and update of the human aldehyde dehydrogenase (ALDH) gene family.  |  Vasiliou, V. and Nebert, DW. 2005. Hum Genomics. 2: 138-43. PMID: 16004729
7. Tissue- and species-specific expression patterns of class I, III, and IV Adh and Aldh 1 mRNAs in rodent embryos.  |  Westerlund, M., et al. 2005. Cell Tissue Res. 322: 227-36. PMID: 16047160
8. Activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in the sera of patients with breast cancer.  |  Jelski, W., et al. 2006. J Clin Lab Anal. 20: 105-8. PMID: 16721836
9. ALDH1A1 Activity in Tumor-Initiating Cells Remodels Myeloid-Derived Suppressor Cells to Promote Breast Cancer Progression.  |  Liu, C., et al. 2021. Cancer Res. 81: 5919-5934. PMID: 34580061
10. Targeting S100A9-ALDH1A1-Retinoic Acid Signaling to Suppress Brain Relapse in EGFR-Mutant Lung Cancer.  |  Biswas, AK., et al. 2022. Cancer Discov. 12: 1002-1021. PMID: 35078784
11. ALDH1A1 drives prostate cancer metastases and radioresistance by interplay with AR- and RAR-dependent transcription.  |  Gorodetska, I., et al. 2024. Theranostics. 14: 714-737. PMID: 38169509
12. Isolation and characterization of aldehyde dehydrogenase isozymes from usual and atypical human livers.  |  Ikawa, M., et al. 1983. J Biol Chem. 258: 6282-7. PMID: 6189823

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (H-4) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (H-4) Specifications

Alternate Product Name: ALDH1A1

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 105-137

Epitope Target: ALDH1A1

Epitope Species: human

Product Reactivity/Detection: ALDH1A1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Aldehyde dehydrogenase /2/ALDH1A1/2/3/ALDH2 antibody is Aldehyde dehydrogenase /2/ALDH1A1/2/3/ALDH2 Antibody (H-8): sc-166362.