Arp2 Antibody (E-12): sc-166103
- Arp2 Antibody (E-12) is a mouse monoclonal IgG1 κ Arp2 antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 311-394 of Arp2 of human origin
- Arp2 Antibody (E-12) is recommended for detection of Arp2 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-Arp2 Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Arp2 Antibody (E-12) for WB and IHC(P) applications. These reagents are now offered in bundles with Arp2 Antibody (E-12) (see ordering information below).
Arp2 Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Arp2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Arp2 (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Arp2 protein plays a crucial role in actin polymerization, which is essential for numerous cellular functions such as chemotaxis, cell migration, cell adhesion, and platelet activation. Arp2 is a key component of the Arp2/3 complex, which facilitates the nucleation of new actin filaments and promotes the formation of a branched actin network. This process is vital for maintaining cellular structure and enabling dynamic changes in cell shape and movement. The Arp2/3 complex operates by binding to the sides of existing actin filaments or the pointed ends, thereby catalyzing the growth of new filaments in the barbed direction. The activity of the Arp2/3 complex is regulated by small GTPases from the Rho family, such as Cdc42, which, upon activation, recruits WASP proteins that further stimulate the complex to initiate the formation of lamellipodia and filopodia. This intricate regulation underscores Arp2′s importance in orchestrating the cytoskeletal dynamics necessary for various cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Arp2 Antibody (E-12) References:
- Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after epidermal growth factor stimulation. | Bailly, M., et al. 1999. J Cell Biol. 145: 331-45. PMID: 10209028
- Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia. | Svitkina, TM. and Borisy, GG. 1999. J Cell Biol. 145: 1009-26. PMID: 10352018
- Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility. | Egile, C., et al. 1999. J Cell Biol. 146: 1319-32. PMID: 10491394
- Regulation of actin polymerization by Arp2/3 complex and WASp/Scar proteins. | Higgs, HN. and Pollard, TD. 1999. J Biol Chem. 274: 32531-4. PMID: 10551802
- Control of actin assembly and disassembly at filament ends. | Cooper, JA. and Schafer, DA. 2000. Curr Opin Cell Biol. 12: 97-103. PMID: 10679358
- GRB2 links signaling to actin assembly by enhancing interaction of neural Wiskott-Aldrich syndrome protein (N-WASp) with actin-related protein (ARP2/3) complex. | Carlier, MF., et al. 2000. J Biol Chem. 275: 21946-52. PMID: 10781580
- The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments. | Mullins, RD., et al. 1998. Proc Natl Acad Sci U S A. 95: 6181-6. PMID: 9600938
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Arp2 Antibody (E-12) | sc-166103 | 200 µg/ml | $316.00 | |||
Arp2 Antibody (E-12): m-IgG Fc BP-HRP Bundle | sc-528906 | 200 µg Ab; 10 µg BP | $354.00 | |||
Arp2 Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-521508 | 200 µg Ab, 40 µg BP | $354.00 | |||
Arp2 Antibody (E-12): m-IgG1 BP-HRP Bundle | sc-543202 | 200 µg Ab; 20 µg BP | $354.00 | |||
Arp2 Antibody (E-12) AC | sc-166103 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Arp2 Antibody (E-12) HRP | sc-166103 HRP | 200 µg/ml | $316.00 | |||
Arp2 Antibody (E-12) FITC | sc-166103 FITC | 200 µg/ml | $330.00 | |||
Arp2 Antibody (E-12) PE | sc-166103 PE | 200 µg/ml | $343.00 | |||
Arp2 Antibody (E-12) Alexa Fluor® 488 | sc-166103 AF488 | 200 µg/ml | $357.00 | |||
Arp2 Antibody (E-12) Alexa Fluor® 546 | sc-166103 AF546 | 200 µg/ml | $357.00 | |||
Arp2 Antibody (E-12) Alexa Fluor® 594 | sc-166103 AF594 | 200 µg/ml | $357.00 | |||
Arp2 Antibody (E-12) Alexa Fluor® 647 | sc-166103 AF647 | 200 µg/ml | $357.00 | |||
Arp2 Antibody (E-12) Alexa Fluor® 680 | sc-166103 AF680 | 200 µg/ml | $357.00 | |||
Arp2 Antibody (E-12) Alexa Fluor® 790 | sc-166103 AF790 | 200 µg/ml | $357.00 |