Clb3 Antibody (C-2): sc-136983
- Clb3 Antibody (C-2) is a mouse monoclonal IgG1 κ Clb3 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-427 mapping near the N-terminus of Clb3 of Saccharomyces cerevisiae origin
- Clb3 Antibody (C-2) is recommended for detection of Clb3 of y origin by WB, IP, IF and ELISA
- Anti-Clb3 Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Clb3 Antibody (C-2). This work is in progress.
QUICK LINKS
Clb3 Antibody (C-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Clb3 of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Clb3 antibody (C-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Clb3 plays a crucial role in cell cycle regulation, particularly during G1 to S phase transition, where Clb3 associates with cyclin-dependent protein kinase Cdc28. This interaction ensures cells only replicate DNA when ready, preventing errors that could lead to genomic instability. Clb3 expression, along with other cyclins, undergoes tight regulation through ubiquitin-mediated proteolysis, mediated by proteins such as Ubc9 and Cdc34. Such regulation maintains precise cell cycle event timing and coordinated cellular processes, highlighting Clb3′s importance in cellular proliferation and overall organismal health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Clb3 Antibody (C-2) References:
- Negative regulation of G1 and G2 by S-phase cyclins of Saccharomyces cerevisiae. | Basco, RD., et al. 1995. Mol Cell Biol. 15: 5030-42. PMID: 7651421
- Increased ubiquitin expression suppresses the cell cycle defect associated with the yeast ubiquitin conjugating enzyme, CDC34 (UBC3). Evidence for a noncovalent interaction between CDC34 and ubiquitin. | Prendergast, JA., et al. 1995. J Biol Chem. 270: 9347-52. PMID: 7721857
- Role of a ubiquitin-conjugating enzyme in degradation of S- and M-phase cyclins. | Seufert, W., et al. 1995. Nature. 373: 78-81. PMID: 7800043
- Starting to cycle: G1 controls regulating cell division in budding yeast. | Sherlock, G. and Rosamond, J. 1993. J Gen Microbiol. 139: 2531-41. PMID: 8277239
- Mechanisms that help the yeast cell cycle clock tick: G2 cyclins transcriptionally activate G2 cyclins and repress G1 cyclins. | Amon, A., et al. 1993. Cell. 74: 993-1007. PMID: 8402888
- Control of the yeast cell cycle by the Cdc28 protein kinase. | Nasmyth, K. 1993. Curr Opin Cell Biol. 5: 166-79. PMID: 8507488
- G2 cyclins are required for the degradation of G1 cyclins in yeast. | Blondel, M. and Mann, C. 1996. Nature. 384: 279-82. PMID: 8918881
- Saccharomyces cerevisiae G1 cyclins differ in their intrinsic functional specificities. | Levine, K., et al. 1996. Mol Cell Biol. 16: 6794-803. PMID: 8943334
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Clb3 Antibody (C-2) | sc-136983 | 200 µg/ml | $316.00 | |||
Clb3 Antibody (C-2) AC | sc-136983 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Clb3 Antibody (C-2) HRP | sc-136983 HRP | 200 µg/ml | $316.00 | |||
Clb3 Antibody (C-2) FITC | sc-136983 FITC | 200 µg/ml | $330.00 | |||
Clb3 Antibody (C-2) PE | sc-136983 PE | 200 µg/ml | $343.00 | |||
Clb3 Antibody (C-2) Alexa Fluor® 488 | sc-136983 AF488 | 200 µg/ml | $357.00 | |||
Clb3 Antibody (C-2) Alexa Fluor® 546 | sc-136983 AF546 | 200 µg/ml | $357.00 | |||
Clb3 Antibody (C-2) Alexa Fluor® 594 | sc-136983 AF594 | 200 µg/ml | $357.00 | |||
Clb3 Antibody (C-2) Alexa Fluor® 647 | sc-136983 AF647 | 200 µg/ml | $357.00 | |||
Clb3 Antibody (C-2) Alexa Fluor® 680 | sc-136983 AF680 | 200 µg/ml | $357.00 | |||
Clb3 Antibody (C-2) Alexa Fluor® 790 | sc-136983 AF790 | 200 µg/ml | $357.00 |