EWS Antibody (C-9): sc-48404

EWS Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects EWS in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). EWS Antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EWS is a crucial nuclear RNA-binding protein that plays a significant role in gene regulation and cellular processes. Notably, EWS gene is often involved in chromosomal translocations that lead to the formation of fusion proteins with transcription factors, such as ATF-1, which are implicated in human neoplasias. In Ewing sarcoma, EWS protein is fused to the DNA-binding domain of ETS transcription factors, including Fli-1, Erg, ETV1, E1AF, and FEV, resulting in the EWS/Fli-1 chimeric protein. This fusion protein acts as a potent transcriptional activator, enhancing the expression of target genes and promoting cell transformation, which is critical for tumorigenesis. EWS protein contains two functional regions: an amino-terminal region (domain A) that has limited transactivation activity but can efficiently induce transformation when fused to Fli-1, and a distal region (domain B) that exhibits transactivation activity but is less effective in promoting transformation when associated with Fli-1. Understanding the functional dynamics of EWS and its interactions is essential for elucidating its role in cancer biology and developing targeted therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EWS Antibody (C-9) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-EWS Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EWS Antibody (C-9) Specifications

Alternate Product Name: EWSR1

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-19

Epitope Target: EWS

Epitope Species: human

Product Reactivity/Detection: EWS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for EWS Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred EWS antibody is EWS Antibody (G-5): sc-28327.