Heme Oxygenase 1/HMOX1 Antibody (A-3): sc-136960

Heme Oxygenase 1 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Heme Oxygenase 1 (HO-1) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HO-1 antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HO-1, also known as HSP 32, plays a crucial role in cellular defense mechanisms by catalyzing the degradation of heme into biliverdin, carbon monoxide, and free iron, thereby providing antioxidant protection and regulating inflammation. The production of carbon monoxide by HO-1 is particularly significant as a signaling molecule that can modulate various physiological processes, including vasodilation and neurotransmission. HO-1 is highly inducible in response to stressors such as heavy metals, hormones, and oxidative stress, making HO-1 a key player in the cellular response to injury and inflammation. In contrast, Heme Oxygenase 2 is constitutively expressed in mammalian tissues and is primarily involved in carbon monoxide production in the brain, where carbon monoxide is thought to function as a neurotransmitter. Understanding HO-1 function is essential for exploring potential therapeutic applications in conditions characterized by oxidative stress and inflammation.

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Heme Oxygenase 1/HMOX1 Antibody (A-3) References:

1. Developmental biology of heme oxygenase.  |  Rodgers, PA. and Stevenson, DK. 1990. Clin Perinatol. 17: 275-91. PMID: 2196131
2. Heme oxygenase: function, multiplicity, regulatory mechanisms, and clinical applications.  |  Maines, MD. 1988. FASEB J. 2: 2557-68. PMID: 3290025
3. Isolation and characterization of the mouse heme oxygenase-1 gene. Distal 5' sequences are required for induction by heme or heavy metals.  |  Alam, J., et al. 1994. J Biol Chem. 269: 1001-9. PMID: 8288554
4. The heme oxygenase system: a regulator of second messenger gases.  |  Maines, MD. 1997. Annu Rev Pharmacol Toxicol. 37: 517-54. PMID: 9131263
5. Isolation and characterization of a cDNA from the rat brain that encodes hemoprotein heme oxygenase-3.  |  McCoubrey, WK., et al. 1997. Eur J Biochem. 247: 725-32. PMID: 9266719
6. Nitric oxide and carbon monoxide: parallel roles as neural messengers.  |  Snyder, SH., et al. 1998. Brain Res Brain Res Rev. 26: 167-75. PMID: 9651518
7. Heme oxygenase-1-derived carbon monoxide contributes to the suppression of acute hypertensive responses in vivo.  |  Motterlini, R., et al. 1998. Circ Res. 83: 568-77. PMID: 9734480

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Heme Oxygenase 1/HMOX1 Antibody (A-3) has 260 citations in a variety of scientific publications.

Primary antibodies like Anti-Heme Oxygenase 1/HMOX1 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Heme Oxygenase 1/HMOX1 Antibody (A-3) Specifications

Alternate Product Name: Heme Oxygenase 1

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 184-288

Epitope Target: Heme Oxygenase 1

Epitope Species: human

Product Reactivity/Detection: Heme Oxygenase 1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 260 citations in various scientific publications

Available Conjugates/Forms for Heme Oxygenase 1/HMOX1 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin