HNF-3β Antibody (RY-7): sc-101060

HNF-3β Antibody (RY-7) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect Hepatocyte Nuclear Factor 3-beta (HNF-3β), also known as FOXA2, in mouse, rat, and human samples using western blotting (WB), immunoprecipitation (IP), PAR assays, and enzyme-linked immunosorbent assay (ELISA) and is raised against recombinant human HNF-3β. HNF-3β is a pivotal member of the forkhead box (FOX) transcription factor family, playing a crucial role in regulating gene expression during both embryonic development and in adult tissues. As a pioneer transcription factor, HNF-3β can bind to condensed chromatin, thereby facilitating the access of other transcription factors and influencing the development and differentiation of essential organs such as the liver, pancreas, and lungs. HNF-3β is indispensable for endoderm formation during gastrulation and plays a significant role in maintaining metabolism and glucose homeostasis. Dysregulation of HNF-3β has been linked to various developmental disorders and diseases, including diabetes and certain cancers, highlighting its importance in both normal physiology and disease pathogenesis. HNF-3β (RY-7) antibody is available for multiple applications, including immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP), making this antibody a versatile tool for researchers studying the function and regulation of HNF-3β. By utilizing anti-HNF-3β antibody (RY-7), scientists can gain deeper insights into the molecular mechanisms governing organ development, metabolic regulation, and the underlying causes of related diseases, thereby advancing our understanding and potential therapeutic approaches.

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HNF-3β Antibody (RY-7) References:

1. Hepatic nuclear factor-3 (HNF-3 or Foxa2) regulates glucagon gene transcription by binding to the G1 and G2 promoter elements.  |  Gauthier, BR., et al. 2002. Mol Endocrinol. 16: 170-83. PMID: 11773447
2. Adenovirus-mediated increase in HNF-3beta or HNF-3alpha shows differences in levels of liver glycogen and gene expression.  |  Tan, Y., et al. 2002. Hepatology. 35: 30-9. PMID: 11786957
3. HNF-3beta is a critical factor for the expression of the Jaagsiekte sheep retrovirus long terminal repeat in type II pneumocytes but not in Clara cells.  |  McGee-Estrada, K., et al. 2002. Virology. 292: 87-97. PMID: 11878911
4. Elevated hepatocyte levels of the Forkhead box A2 (HNF-3beta) transcription factor cause postnatal steatosis and mitochondrial damage.  |  Hughes, DE., et al. 2003. Hepatology. 37: 1414-24. PMID: 12774021
5. Transcription factor HNF-6/OC-1 inhibits the stimulation of the HNF-3alpha/Foxa1 gene by TGF-beta in mouse liver.  |  Plumb-Rudewiez, N., et al. 2004. Hepatology. 40: 1266-74. PMID: 15562441
6. More potent transcriptional activators or a transdominant inhibitor of the HNF1 homeoprotein family are generated by alternative RNA processing.  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
7. The HNF-3 gene family of transcription factors in mice: gene structure, cDNA sequence, and mRNA distribution.  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
8. Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes.  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
9. The transcriptional activator hepatocyte nuclear factor 6 regulates liver gene expression.  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HNF-3β Antibody (RY-7) has 61 citations in a variety of scientific publications.

Primary antibodies like Anti-HNF-3β Antibody (RY-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HNF-3β Antibody (RY-7) Specifications

Alternate Product Name: FOXA2

Product Clone Name: RY-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 363-458

Epitope Target: HNF-3β

Epitope Species: human

Product Reactivity/Detection: HNF-3β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 61 citations in various scientific publications

Available Conjugates/Forms for HNF-3β Antibody (RY-7): N/A