HSP 10 Antibody (D-8): sc-376313

HSP 10 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSP 10 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HSP 10 antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HSP 10, a member of the heat shock protein family, plays a crucial role in cellular stress responses by assisting in proper protein folding and preventing aggregation, which maintains cellular homeostasis, especially under stress conditions such as heat shock or oxidative stress. HSP 10 is primarily located in mitochondria, where HSP 10 forms a complex with HSP 60, facilitating import and folding of mitochondrial proteins. HSP 10 interaction with HSP 60 is essential for mitochondrial function, ensuring newly synthesized proteins are correctly folded and functional, thereby supporting cellular energy metabolism and preventing mitochondrial dysfunction, which can lead to various diseases. HSP 10 (D-8) monoclonal antibody serves as an invaluable tool for researchers studying molecular chaperones in health and disease, particularly in mitochondrial biology and stress responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HSP 10 Antibody (D-8) References:

1. HSP-10 in ovarian cancer: expression and suppression of T-cell signaling.  |  Akyol, S., et al. 2006. Gynecol Oncol. 101: 481-6. PMID: 16386781
2. The universally conserved GroE (Hsp60) chaperonins.  |  Zeilstra-Ryalls, J., et al. 1991. Annu Rev Microbiol. 45: 301-25. PMID: 1683763
3. Role of heat shock protein 70 in hepatic ischemia-reperfusion injury in mice.  |  Kuboki, S., et al. 2007. Am J Physiol Gastrointest Liver Physiol. 292: G1141-9. PMID: 17185630
4. Inhibitor of heat-shock protein 90 enhances the antitumor effect of DNA vaccine targeting clients of heat-shock protein.  |  Lin, CC., et al. 2007. Mol Ther. 15: 404-10. PMID: 17235320
5. Interleukin-6, interleukin-10 and heat shock protein-90 expression in renal epithelial neoplasias and surrounding normal-appearing renal parenchyma.  |  Cardillo, MR. and Ippoliti, F. 2007. Int J Immunopathol Pharmacol. 20: 37-46. PMID: 17346426
6. Differential recognition of heat-shock protein dnaJ-derived epitopes by effector and Treg cells leads to modulation of inflammation in juvenile idiopathic arthritis.  |  Massa, M., et al. 2007. Arthritis Rheum. 56: 1648-57. PMID: 17469159
7. Human Hsp10 and Early Pregnancy Factor (EPF) and their relationship and involvement in cancer and immunity: current knowledge and perspectives.  |  Corrao, S., et al. 2010. Life Sci. 86: 145-52. PMID: 19913561
8. Disease-Associated Mutations in the HSPD1 Gene Encoding the Large Subunit of the Mitochondrial HSP60/HSP10 Chaperonin Complex.  |  Bross, P. and Fernandez-Guerra, P. 2016. Front Mol Biosci. 3: 49. PMID: 27630992
9. Evolution of the chaperonin families (Hsp60, Hsp10 and Tcp-1) of proteins and the origin of eukaryotic cells.  |  Gupta, RS. 1995. Mol Microbiol. 15: 1-11. PMID: 7752884
10. Dynamics of the chaperonin ATPase cycle: implications for facilitated protein folding.  |  Todd, MJ., et al. 1994. Science. 265: 659-66. PMID: 7913555
11. Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ.  |  Ohtsuka, K. 1993. Biochem Biophys Res Commun. 197: 235-40. PMID: 8250930
12. Role of the major heat shock proteins as molecular chaperones.  |  Georgopoulos, C. and Welch, WJ. 1993. Annu Rev Cell Biol. 9: 601-34. PMID: 8280473

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSP 10 Antibody (D-8) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-HSP 10 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSP 10 Antibody (D-8) Specifications

Alternate Product Name: HSP 10

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-102

Epitope Target: HSP 10

Epitope Species: human

Product Reactivity/Detection: HSP 10

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for HSP 10 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin