Phox2b Antibody (C-3): sc-376993

Phox2b Antibody (C-3) is a mouse monoclonal IgG2a antibody that detects Phox2b in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Phox2b is a critical transcription factor that plays a vital role in the development and differentiation of the enteric nervous system, as well as in the formation of sympathetic and parasympathetic neurons. Phox2b is predominantly located in the nucleus of neuronal cells, where Phox2b regulates gene expression essential for neuronal identity and function. Nuclear localization of Phox2b is crucial because Phox2b interacts with specific DNA sequences, thereby influencing the transcription of target genes involved in neuronal differentiation. Furthermore, Phox2b is essential for the differentiation of noradrenergic neurons in both central and peripheral nervous systems, making Phox2b a key player in the development of the autonomic nervous system. Phox2b promotes the expression of downstream genes, such as tyrosine hydroxylase and dopamine-beta hydroxylase, underscoring Phox2b′s importance in neurotransmitter synthesis and neuronal signaling. With a significant role in neuronal development and function, Phox2b is a valuable target for research into neurodevelopmental disorders and related pathologies.

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Phox2b Antibody (C-3) References:

1. Phox2b, RTN/pFRG neurons and respiratory rhythmogenesis.  |  Onimaru, H., et al. 2009. Respir Physiol Neurobiol. 168: 13-8. PMID: 19712902
2. Distinctive features of Phox2b-expressing neurons in the rat reticular formation dorsal to the trigeminal motor nucleus.  |  Nagoya, K., et al. 2017. Neuroscience. 358: 211-226. PMID: 28673717
3. Causative and common PHOX2B variants define a broad phenotypic spectrum.  |  Bachetti, T. and Ceccherini, I. 2020. Clin Genet. 97: 103-113. PMID: 31444792
4. Respiratory Control by Phox2b-expressing Neurons in a Locus Coeruleus-preBötzinger Complex Circuit.  |  Liu, N., et al. 2021. Neurosci Bull. 37: 31-44. PMID: 32468398
5. PHOX2B as a Reliable Marker for Neuroblastoma in Tissue and Cytology Specimens.  |  Ma, Y., et al. 2021. J Neuropathol Exp Neurol. 80: 1108-1116. PMID: 34965292
6. PHOX2B is a Sensitive and Specific Marker for the Histopathological Diagnosis of Pheochromocytoma and Paraganglioma.  |  Miyauchi, M., et al. 2022. Endocr Pathol. 33: 506-518. PMID: 36029394
7. Phox2B is a sensitive and reliable marker of paraganglioma-Phox2B immunohistochemistry in diagnosis of neuroendocrine neoplasms.  |  Manethova, M., et al. 2023. Virchows Arch. 482: 679-686. PMID: 36656393
8. Organoid models of breathing disorders reveal patterning defect of hindbrain neurons caused by PHOX2B-PARMs.  |  Lui, KN., et al. 2023. Stem Cell Reports. 18: 1500-1515. PMID: 37352849
9. Phox2b-expressing neurons contribute to breathing problems in Kcnq2 loss- and gain-of-function encephalopathy models.  |  Soto-Perez, J., et al. 2023. Nat Commun. 14: 8059. PMID: 38052789
10. Knockdown of PHOX2B in the retrotrapezoid nucleus reduces the central CO2 chemoreflex in rats.  |  Cardani, S., et al. 2024. Elife. 13: PMID: 38727716

The preferred Phox2b antibody is Phox2b Antibody (B-11): sc-376997.