Sp1 Antibody (1C6): sc-420

Sp1 Antibody (1C6) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 609-627 mapping to an internal domain of human-origin Specificity Protein 1 (Sp1), a transcription factor involved in the regulation of numerous genes. Sp1 monoclonal antibody (1C6) reacts with mouse, rat, and human species and is suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Sp1, also known as Transcription Factor Sp1, is a zinc finger protein that binds to GC-rich motifs in DNA, commonly referred to as GC boxes, playing a crucial role in gene expression, cell growth, differentiation, and apoptosis. Initially identified as a factor that activates transcription from the SV40 promoter by binding to multiple GC boxes in the 21-base pair repeats, Sp1′s DNA-binding specificity is conferred by three Cys2His2-type zinc finger motifs. Phosphorylation of Sp1 can modulate activity and DNA-binding affinity, affecting the transcription of target genes. Located predominantly in the nucleus, Sp1′s localization is essential for function as a transcriptional regulator. Dysregulation of Sp1 has been implicated in various diseases, including cancer, highlighting importance in maintaining normal cellular functions. Sp1 monoclonal antibody (1C6) enables researchers to study the expression and function of Sp1 in different biological contexts, aiding in the understanding of its role in health and disease.

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Sp1 Antibody (1C6) References:

1. Functional significance of an overlapping consensus binding motif for Sp1 and Zif268 in the murine adenosine deaminase gene promoter.  |  Ackerman, SL., et al. 1991. Proc Natl Acad Sci U S A. 88: 7523-7. PMID: 1881892
2. GC box binding induces phosphorylation of Sp1 by a DNA-dependent protein kinase.  |  Jackson, SP., et al. 1990. Cell. 63: 155-65. PMID: 2170018
3. Distinct regions of Sp1 modulate DNA binding and transcriptional activation.  |  Kadonaga, JT., et al. 1988. Science. 242: 1566-70. PMID: 3059495
4. Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.  |  Kadonaga, JT., et al. 1987. Cell. 51: 1079-90. PMID: 3319186
5. Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.  |  Dynan, WS. and Tjian, R. 1983. Cell. 32: 669-80. PMID: 6187469
6. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.  |  Dynan, WS. and Tjian, R. 1983. Cell. 35: 79-87. PMID: 6313230
7. Localization of the human Sp1 transcription factor gene to 12q13 by fluorescence in situ hybridization.  |  Matera, AG. and Ward, DC. 1993. Genomics. 17: 793-4. PMID: 8244404

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sp1 Antibody (1C6) has 358 citations in a variety of scientific publications.

Primary antibodies like Anti-Sp1 Antibody (1C6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sp1 Antibody (1C6) Specifications

Alternate Product Name: Sp1

Product Clone Name: 1C6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 609-627

Epitope Target: Sp1

Epitope Species: human

Product Reactivity/Detection: Sp1 p95 and p106

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 358 citations in various scientific publications

Available Conjugates/Forms for Sp1 Antibody (1C6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Sp1 antibody is Sp1 Antibody (E-3): sc-17824.