Date published: 2025-11-25

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SP600125 (CAS 129-56-6)

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Alternate Names:
JNK Inhibitor II; Anthra(1,9-cd)pyrazol-6(2H)-one
Application:
SP600125 is a potent, selective and reversible inhibitor of JNK1, JNK2 and JNK3.
CAS Number:
129-56-6
Purity:
≥97%
Molecular Weight:
220.23
Molecular Formula:
C14H8N2O
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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SP600125 is a potent, selective and reversible inhibitor of the three JNK enzymes (c-Jun N-terminal kinases), with IC50 = 40 nM for JNK1 and JNK2, and 90 nM for JNK3. Inhibition is competitive with respect to ATP, and over 300-fold more selective for JNK as compared to ERK1 and p38-2 MAP kinases. SP600125 was shown to cause a dose-dependent inhibition of the phosphorylation of c-Jun, and thereby the expression of inflammatory genes COX-2, IFN-gamma, IL-2, TNF-alpha, and block the activation and differentiation of primary human CD4 cell cultures. SP 600125 has also been shown to prevent apoptosis in an array of cell types, and inhibit autophagy in HeLa cells.


SP600125 (CAS 129-56-6) References

  1. c-Jun N-terminal kinase is required for metalloproteinase expression and joint destruction in inflammatory arthritis.  |  Han, Z., et al. 2001. J Clin Invest. 108: 73-81. PMID: 11435459
  2. SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase.  |  Bennett, BL., et al. 2001. Proc Natl Acad Sci U S A. 98: 13681-6. PMID: 11717429
  3. SP600125, a new JNK inhibitor, protects dopaminergic neurons in the MPTP model of Parkinson's disease.  |  Wang, W., et al. 2004. Neurosci Res. 48: 195-202. PMID: 14741394
  4. Cross-linking of B7-H1 on EBV-transformed B cells induces apoptosis through reactive oxygen species production, JNK signaling activation, and fasL expression.  |  Kim, YS., et al. 2008. J Immunol. 181: 6158-69. PMID: 18941206
  5. SP600125 inhibits cap-dependent translation independently of the c-Jun N-terminal kinase pathway.  |  Ito, M., et al. 2011. Cell Struct Funct. 36: 27-33. PMID: 21263197
  6. SP600125 induces Src and type I IGF receptor phosphorylation independent of JNK.  |  Kong, Q., et al. 2014. Int J Mol Sci. 15: 16246-56. PMID: 25226534
  7. SP600125 suppresses Keap1 expression and results in NRF2-mediated prevention of diabetic nephropathy.  |  Zhang, H., et al. 2018. J Mol Endocrinol. 60: 145-157. PMID: 29273684
  8. SP600125 enhances C-2-induced cell death by the switch from autophagy to apoptosis in bladder cancer cells.  |  Yu, H., et al. 2019. J Exp Clin Cancer Res. 38: 448. PMID: 31685029
  9. Inhibition of inflammation by SP600125 in cholestatic liver injury is dependent on the administration‑based exposure profile.  |  Zheng, X., et al. 2020. Int J Mol Med. 46: 2271-2279. PMID: 33125089
  10. Protective effects of SP600125 on mice infected with H1N1 influenza A virus.  |  Tang, Y., et al. 2021. Arch Virol. 166: 2151-2158. PMID: 34014386
  11. SP600125 Restored TNF-α-Induced Impaired Chondrogenesis in Bone Mesenchymal Stem Cells and Its Antiosteoarthritis Effect in Mice.  |  Ding, N., et al. 2021. Stem Cells Dev. 30: 1028-1036. PMID: 34486378
  12. SP600125 Enhances Temperature-Controlled Repeated Thermal Stimulation-Induced Neurite Outgrowth in PC12-P1F1 Cells.  |  Luo, YR., et al. 2022. Int J Mol Sci. 23: PMID: 36555248

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

SP600125, 10 mg

sc-200635
10 mg
$40.00

SP600125, 50 mg

sc-200635A
50 mg
$150.00

How long can this product be stored in a DMSO solution?

Asked by: SCM4
Thank you for your question. Long term storage at -20 ºC in DMSO is acceptable for this product.
Answered by: Technical Support
Date published: 2017-03-27

Any recommendations for solubility in DMSO?

Asked by: chemicalsmg
The solubility of SP600125 is 100 mM in DMSO. We recommend rapid stirring, sonication or gentle warming (in a 45-60°C water bath).
Answered by: Tech Service 11
Date published: 2017-03-06
  • y_2025, m_11, d_13, h_10CST
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Rated 5 out of 5 by from works well!Good in lower concentration; higher concentration significantly inhibits Erk signalling. Long lasting inhibition of JNK – checked up to 24 hrs.
Date published: 2017-02-07
Rated 5 out of 5 by from We used this in different human cell linesWe used this in different human cell lines and got good results.
Date published: 2015-10-16
Rated 5 out of 5 by from HepG2HepG2, Hep3B and PLC/PRF/5 cells were pre-treated with SP600125 (from SCBT) before exposure to galangin in order to further characterize galangin's involvement in endoplasmic reticulum stress.
Date published: 2015-02-27
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SP600125 is rated 5.0 out of 5 by 3.
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