Stat3 Antibody (F-2): sc-8019

Stat3 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Stat3 of mouse, rat, and human origin through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). Stat3 monoclonal antibody (F-2) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Stat3 plays a crucial role in mediating cellular responses to a variety of extracellular signals, particularly in the context of growth factor and cytokine signaling. Upon activation by ligands such as interferons and growth hormones, Stat3 undergoes tyrosine phosphorylation, which is essential for translocation to the nucleus where Stat3 regulates gene expression involved in cell proliferation, survival, and differentiation. This function is particularly important in the context of cancer, as aberrant Stat3 activation is often associated with tumorigenesis and poor prognosis. Stat3 can localize to both the nucleus and cytoplasm, and dynamic localization is critical for function in signaling pathways. The ability of Stat3 to interact with other proteins, such as Stat1 and Stat5, further underscores its role in complex signaling networks that govern cellular responses to environmental cues. Stat3 monoclonal antibody (F-2) is an invaluable resource for studying Stat3′s critical functions in signal transduction, gene regulation, and implications in cancer and other cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Stat3 Antibody (F-2) References:

1. Activated STAT3 as a correlate of distant metastasis in prostate cancer: a secondary analysis of Radiation Therapy Oncology Group 86-10.  |  Torres-Roca, JF., et al. 2007. Urology. 69: 505-9. PMID: 17382154
2. The role of STAT3 in autophagy.  |  You, L., et al. 2015. Autophagy. 11: 729-39. PMID: 25951043
3. STAT3 signaling in immunity.  |  Hillmer, EJ., et al. 2016. Cytokine Growth Factor Rev. 31: 1-15. PMID: 27185365
4. Targeting STAT3 in Cancer Immunotherapy.  |  Zou, S., et al. 2020. Mol Cancer. 19: 145. PMID: 32972405
5. Tyrosine-phosphorylated Stat1 and Stat2 plus a 48-kDa protein all contact DNA in forming interferon-stimulated-gene factor 3.  |  Qureshi, SA., et al. 1995. Proc Natl Acad Sci U S A. 92: 3829-33. PMID: 7537377
6. Cooperative transcriptional activity of Jun and Stat3 beta, a short form of Stat3.  |  Schaefer, TS., et al. 1995. Proc Natl Acad Sci U S A. 92: 9097-101. PMID: 7568080
7. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway.  |  Schindler, C. and Darnell, JE. 1995. Annu Rev Biochem. 64: 621-51. PMID: 7574495
8. Interleukin-3, erythropoietin, and prolactin activate a STAT5-like factor in lymphoid cells.  |  Pallard, C., et al. 1995. J Biol Chem. 270: 15942-5. PMID: 7608147
9. Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation.  |  Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
10. An interleukin-4-induced transcription factor: IL-4 Stat.  |  Hou, J., et al. 1994. Science. 265: 1701-6. PMID: 8085155
11. Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6.  |  Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
12. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins.  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Stat3 Antibody (F-2) has 543 citations in a variety of scientific publications.

Primary antibodies like Anti-Stat3 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Stat3 Antibody (F-2) Specifications

Alternate Product Name: Stat3

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 50-240

Epitope Target: Stat3

Epitope Species: human

Product Reactivity/Detection: Stat3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 543 citations in various scientific publications

Available Conjugates/Forms for Stat3 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred p-Stat3 antibody is p-Stat3 Antibody (B-7): sc-8059.