Su[fu] Antibody (F-4): sc-137014

Su(fu) Antibody (F-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Su(fu) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Su(fu) (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Su(fu), known as Suppressor-of-Fused, plays a crucial role as a negative regulator in the vertebrate Hedgehog signaling pathway, which is essential for proper embryonic development and tissue patterning. Su(fu) functions by inhibiting the activity of the transcription factor Gli1 and interacting with Gli2, Gli3, and the serine/threonine kinase Fused, thereby modulating the pathway′s signaling output. This regulation is vital, as aberrations in Hedgehog signaling can lead to various cancers, including glioblastomas, prostate cancer, malignant melanoma, and endometrial cancer, with Su(fu) gene locus often found deleted in these malignancies. Furthermore, Su(fu) is widely expressed in both adult and embryonic tissues, particularly in those influenced by Hedgehog signaling, highlighting Su(fu)′s importance in developmental biology and cancer research.

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Su[fu] Antibody (F-4) References:

1. Hedgehog controls limb development by regulating the activities of distinct transcriptional activator and repressor forms of Cubitus interruptus.  |  Méthot, N. and Basler, K. 1999. Cell. 96: 819-31. PMID: 10102270
2. Vertebrate homologs of Drosophila suppressor of fused interact with the gli family of transcriptional regulators.  |  Pearse, RV., et al. 1999. Dev Biol. 212: 323-36. PMID: 10433824
3. Mammalian suppressor-of-fused modulates nuclear-cytoplasmic shuttling of Gli-1.  |  Kogerman, P., et al. 1999. Nat Cell Biol. 1: 312-9. PMID: 10559945
4. Characterization of the human suppressor of fused, a negative regulator of the zinc-finger transcription factor Gli.  |  Stone, DM., et al. 1999. J Cell Sci. 112 (Pt 23): 4437-48. PMID: 10564661
5. Suppressor of fused negatively regulates beta-catenin signaling.  |  Meng, X., et al. 2001. J Biol Chem. 276: 40113-9. PMID: 11477086
6. Rab23 negatively regulates Gli1 transcriptional factor in a Su(Fu)-dependent manner.  |  Chi, S., et al. 2012. Cell Signal. 24: 1222-8. PMID: 22365972
7. Compounds with Potential Activity against Mycobacterium tuberculosis.  |  Sao Emani, C., et al. 2018. Antimicrob Agents Chemother. 62: PMID: 29437626
8. Interplay between CTHRC1 and the SU protein of avian leukosis virus subgroup J (ALV-J) facilitates viral replication.  |  Pang, Y., et al. 2019. Virus Res. 264: 32-39. PMID: 30797826
9. The Effect of Quercetin Nanosuspension on Prostate Cancer Cell Line LNCaP via Hedgehog Signaling Pathway.  |  Mousavi, N., et al. 2021. Rep Biochem Mol Biol. 10: 69-75. PMID: 34277870
10. Catching a Gli-mpse of Hedgehog.  |  Ruiz i Altaba, A. 1997. Cell. 90: 193-6. PMID: 9244291
11. Suppressor of fused links fused and Cubitus interruptus on the hedgehog signalling pathway.  |  Monnier, V., et al. 1998. Curr Biol. 8: 583-6. PMID: 9601642
12. Hedgehog stimulates maturation of Cubitus interruptus into a labile transcriptional activator.  |  Ohlmeyer, JT. and Kalderon, D. Nature. 396: 749-53. PMID: 9874371

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Su[fu] Antibody (F-4) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Su[fu] Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Su[fu] Antibody (F-4) Specifications

Alternate Product Name: Su[fu]

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 185-484

Epitope Target: suppressor of fused

Epitope Species: human

Product Reactivity/Detection: Su[fu] 54 kDa form (484 amino acid splice variant)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Su[fu] Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin