SURF-6 Antibody (A-5): sc-515439
- SURF-6 Antibody (A-5) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 265-310 mapping near the C-terminus of SURF-6 of human origin
- Anti-SURF-6 Antibody (A-5) is recommended for detection of SURF-6 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SURF-6 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SURF-6 Antibody (A-5) for WB applications. These reagents are now offered in bundles with SURF-6 Antibody (A-5) (see ordering information below).
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SURF-6 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects SURF-6 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SURF-6 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SURF-6, or Surfeit locus protein-6, is a 361 amino acid protein predominantly localized to the granular components of the nucleolus, a critical site for ribosome biogenesis. The nucleolus plays a vital role in synthesizing ribosomal RNA and assembling ribosomal subunits, making SURF-6 essential for proper cellular function. SURF-6 expression is tightly regulated throughout the cell cycle, and studies have shown that cells lacking SURF-6 are nonviable in mice, underscoring SURF-6′s importance in ribosome assembly and cell cycle progression. Additionally, human SURF-6 exhibits structural similarities with orthologs in fish and mice, indicating an evolutionarily conserved function across species. The gene encoding SURF-6 is located on chromosome 9 within the Surfeit gene locus, one of the most densely packed gene clusters in the human genome, highlighting SURF-6′s potential significance in genetic regulation and cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SURF-6 Antibody (A-5) References:
- Isolation and genomic analysis of the human surf-6 gene: a member of the Surfeit locus. | Magoulas, C. and Fried, M. 2000. Gene. 243: 115-23. PMID: 10675619
- Cloning and expression of the surfeit locus member Surf-6 during embryogenesis in Xenopus laevis. | Wolff, CM., et al. 2002. DNA Seq. 13: 149-52. PMID: 12391725
- Identification of an evolutionary conserved SURF-6 domain in a family of nucleolar proteins extending from human to yeast. | Polzikov, M., et al. 2005. Biochem Biophys Res Commun. 327: 143-9. PMID: 15629442
- [Properties and functions of a new nucleolar protein, Surf-6, in 3T3 mouse cells]. | Gurchenkov, VV., et al. 2005. Bioorg Khim. 31: 578-85. PMID: 16363129
- Implication of nucleolar protein SURF6 in ribosome biogenesis and preimplantation mouse development. | Romanova, LG., et al. 2006. Biol Reprod. 75: 690-6. PMID: 16855206
- The nucleolar protein SURF-6 is essential for viability in mouse NIH/3T3 cells. | Polzikov, M., et al. 2007. Mol Biol Rep. 34: 155-60. PMID: 17086444
- The Surf-6 gene of the mouse surfeit locus encodes a novel nucleolar protein. | Magoulas, C. and Fried, M. 1996. DNA Cell Biol. 15: 305-16. PMID: 8639267
- The SURF-6 protein is a component of the nucleolar matrix and has a high binding capacity for nucleic acids in vitro. | Magoulas, C., et al. 1998. Eur J Cell Biol. 75: 174-83. PMID: 9548374
- The human Surfeit locus. | Duhig, T., et al. 1998. Genomics. 52: 72-8. PMID: 9740673
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SURF-6 Antibody (A-5) | sc-515439 | 200 µg/ml | $322.00 | |||
SURF-6 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-531367 | 200 µg Ab; 10 µg BP | $361.00 | |||
SURF-6 Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-525040 | 200 µg Ab, 40 µg BP | $361.00 | |||
SURF-6 Antibody (A-5): m-IgG1 BP-HRP Bundle | sc-544508 | 200 µg Ab; 20 µg BP | $361.00 | |||
SURF-6 Antibody (A-5) AC | sc-515439 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SURF-6 Antibody (A-5) HRP | sc-515439 HRP | 200 µg/ml | $322.00 | |||
SURF-6 Antibody (A-5) FITC | sc-515439 FITC | 200 µg/ml | $336.00 | |||
SURF-6 Antibody (A-5) PE | sc-515439 PE | 200 µg/ml | $349.00 | |||
SURF-6 Antibody (A-5) Alexa Fluor® 488 | sc-515439 AF488 | 200 µg/ml | $364.00 | |||
SURF-6 Antibody (A-5) Alexa Fluor® 546 | sc-515439 AF546 | 200 µg/ml | $364.00 | |||
SURF-6 Antibody (A-5) Alexa Fluor® 594 | sc-515439 AF594 | 200 µg/ml | $364.00 | |||
SURF-6 Antibody (A-5) Alexa Fluor® 647 | sc-515439 AF647 | 200 µg/ml | $364.00 | |||
SURF-6 Antibody (A-5) Alexa Fluor® 680 | sc-515439 AF680 | 200 µg/ml | $364.00 | |||
SURF-6 Antibody (A-5) Alexa Fluor® 790 | sc-515439 AF790 | 200 µg/ml | $364.00 |