SA-1 Antibody (A-9): sc-365061
- SA-1 Antibody (A-9) is a mouse monoclonal IgG2a κ SA-1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1117-1202 mapping near the C-terminus of SA-1 of human origin
- SA-1 Antibody (A-9) is recommended for detection of SA-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SA-1 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for SA-1 Antibody (A-9) for WB applications. This reagent is now offered in a bundle with SA-1 Antibody (A-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
SA-1 Antibody (A-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects SA-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SA-1 (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SA-1, also known as STAG1, plays a crucial role in the cohesion complex, which is essential for proper segregation of sister chromatids during cell division. This multi-protein structure maintains chromatid cohesion after DNA replication and participates in mitotic spindle pole assembly. SA-1, a 1258 amino acid protein, interacts directly with Rad21, forming a heterodimer with SMC1 and SMC3, which are linked at their heads by Rad21. Localized primarily in the nucleus, SA-1 associates with chromatin and undergoes phosphorylation by Polo-like kinase (Plk), triggering dissociation from chromatin and facilitating accurate chromosome separation during anaphase. As a human homolog of yeast Scc3p, SA-1 shows predominant expression in thymus, bone marrow, and spleen, exhibiting 99% similarity to its mouse counterpart, highlighting evolutionary conservation and importance in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SA-1 Antibody (A-9) References:
- Increased platinum accumulation in SA-1 tumour cells after in vivo electrochemotherapy with cisplatin. | Cemazar, M., et al. 1999. Br J Cancer. 79: 1386-91. PMID: 10188880
- Characterization of vertebrate cohesin complexes and their regulation in prophase. | Sumara, I., et al. 2000. J Cell Biol. 151: 749-62. PMID: 11076961
- Reduced blood flow and oxygenation in SA-1 tumours after electrochemotherapy with cisplatin. | Sersa, G., et al. 2002. Br J Cancer. 87: 1047-54. PMID: 12434299
- Identification of STAG1 as a key mediator of a p53-dependent apoptotic pathway. | Anazawa, Y., et al. 2004. Oncogene. 23: 7621-7. PMID: 15361841
- Cohesion proteins are present in centromere protein bodies associated with avian lampbrush chromosomes. | Krasikova, A., et al. 2005. Chromosome Res. 13: 675-85. PMID: 16235117
- High level expression of STAG1/PMEPA1 in an androgen-independent prostate cancer PC3 subclone. | Hirokawa, YS., et al. 2007. Cell Mol Biol Lett. 12: 370-7. PMID: 17318295
- Induction of systemic lupus erythematosus in naive mice with T-cell lines specific for human anti-DNA antibody SA-1 (16/6 Id+) and for mouse tuberculosis antibody TB/68 (16/6 Id+). | Blank, M., et al. 1991. Clin Immunol Immunopathol. 60: 471-83. PMID: 1830833
- Inhibition of SA-1 tumor growth in mice by human leukocyte interferon alpha combined with low-level direct current. | Sersa, G. and Miklavcic, D. 1990. Mol Biother. 2: 165-8. PMID: 2222901
- Cohesinopathy presenting with microtia, facial palsy, and hearing loss caused by STAG1 pathogenic variant. | Funato, M., et al. 2022. Congenit Anom (Kyoto). 62: 82-83. PMID: 34914139
- Effects of vinblastine on cell membrane fluidity and the growth of SA-1 tumor in mice. | Sersa, G., et al. 1994. Cancer Lett. 79: 53-60. PMID: 8187053
- Anti-tumor effectiveness of electrochemotherapy with bleomycin is increased by TNF-alpha on SA-1 tumors in mice. | Sersa, G., et al. 1997. Cancer Lett. 116: 85-92. PMID: 9177462
- SA-1, a nuclear protein encoded by one member of a novel gene family: molecular cloning and detection in hemopoietic organs. | Carramolino, L., et al. 1997. Gene. 195: 151-9. PMID: 9305759
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SA-1 Antibody (A-9) | sc-365061 | 200 µg/ml | $322.00 | |||
SA-1 Antibody (A-9): m-IgGκ BP-HRP Bundle | sc-551093 | 200 µg Ab; 40 µg BP | $361.00 | |||
SA-1 Antibody (A-9) AC | sc-365061 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SA-1 Antibody (A-9) HRP | sc-365061 HRP | 200 µg/ml | $322.00 | |||
SA-1 Antibody (A-9) FITC | sc-365061 FITC | 200 µg/ml | $336.00 | |||
SA-1 Antibody (A-9) PE | sc-365061 PE | 200 µg/ml | $349.00 | |||
SA-1 Antibody (A-9) Alexa Fluor® 488 | sc-365061 AF488 | 200 µg/ml | $364.00 | |||
SA-1 Antibody (A-9) Alexa Fluor® 546 | sc-365061 AF546 | 200 µg/ml | $364.00 | |||
SA-1 Antibody (A-9) Alexa Fluor® 594 | sc-365061 AF594 | 200 µg/ml | $364.00 | |||
SA-1 Antibody (A-9) Alexa Fluor® 647 | sc-365061 AF647 | 200 µg/ml | $364.00 | |||
SA-1 Antibody (A-9) Alexa Fluor® 680 | sc-365061 AF680 | 200 µg/ml | $364.00 | |||
SA-1 Antibody (A-9) Alexa Fluor® 790 | sc-365061 AF790 | 200 µg/ml | $364.00 |