ODC Antibody (E-6): sc-398116

ODC Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects ODC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ODC (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ornithine decarboxylase (ODC) is a crucial enzyme that catalyzes the conversion of ornithine to putrescine, marking the first step in polyamine biosynthesis, which is vital for cell growth, differentiation, and apoptosis. ODC regulation is tightly controlled, as ODC overexpression is linked to increased cell proliferation and cancer development. Notably, ODC promoter is influenced by several transcription factors, including Sp1, c-Myc, and c-Fos, which act as transactivators, while ZBP-89 serves as a transrepressor. Furthermore, elevated levels of polyamines can lead to a feedback mechanism that downregulates ODC activity through enhanced translation of antizyme mRNA, which subsequently binds to ODC monomers, targeting them for degradation by the 26S proteasome. ODC gene is located on human chromosome 2p25, and ODC expression is associated with angiogenesis, as ODC-overexpressing cells have been shown to suppress Type XVIII collagen and endostatin expression, thereby promoting endothelial cell proliferation. anti-ODC antibody (E-6) availability in various formats makes this product an essential tool for researchers studying ODC role in cellular processes and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ODC Antibody (E-6) References:

1. ZBP-99 defines a conserved family of transcription factors and regulates ornithine decarboxylase gene expression.  |  Law, DJ., et al. 1999. Biochem Biophys Res Commun. 262: 113-20. PMID: 10448078
2. Crystal structure of human ornithine decarboxylase at 2.1 A resolution: structural insights to antizyme binding.  |  Almrud, JJ., et al. 2000. J Mol Biol. 295: 7-16. PMID: 10623504
3. p53 independent G(1) arrest induced by DL-alpha-difluoromethylornithine.  |  Nemoto, T., et al. 2001. Biochem Biophys Res Commun. 280: 848-54. PMID: 11162600
4. Effects of alpha-difluoromethylornithine on the Fas expression and apoptosis in Hep-2 cells.  |  Alvarez, MG., et al. 2000. Biocell. 24: 213-6. PMID: 11201656
5. The ornithine decarboxylase gene is essential for cell survival during early murine development.  |  Pendeville, H., et al. 2001. Mol Cell Biol. 21: 6549-58. PMID: 11533243
6. Multiple promoter elements govern expression of the human ornithine decarboxylase gene in colon carcinoma cells.  |  Moshier, JA., et al. 1992. Nucleic Acids Res. 20: 2581-90. PMID: 1598217
7. Molecular genetics of polyamine synthesis in eukaryotic cells.  |  Heby, O. and Persson, L. 1990. Trends Biochem Sci. 15: 153-8. PMID: 2187296
8. Polyamine metabolism and its importance in neoplastic growth and a target for chemotherapy.  |  Pegg, AE. 1988. Cancer Res. 48: 759-74. PMID: 3123052
9. Ribonucleotide reductase M2 subunit sequences mapped to four different chromosomal sites in humans and mice: functional locus identified by its amplification in hydroxyurea-resistant cell lines.  |  Yang-Feng, TL., et al. 1987. Genomics. 1: 77-86. PMID: 3311968
10. DMSO-tolerant ornithine decarboxylase (ODC) tandem assay optimised for high-throughput screening.  |  Park, MG., et al. 2023. J Enzyme Inhib Med Chem. 38: 309-318. PMID: 36451618
11. Polyamines.  |  Tabor, CW. and Tabor, H. 1984. Annu Rev Biochem. 53: 749-90. PMID: 6206782
12. Direct transcriptional stimulation of the ornithine decarboxylase gene by Fos in PC12 cells but not in fibroblasts.  |  Wrighton, C. and Busslinger, M. 1993. Mol Cell Biol. 13: 4657-69. PMID: 8101634

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ODC Antibody (E-6) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-ODC Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ODC Antibody (E-6) Specifications

Alternate Product Name: Ornithine Decarboxylase

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 243-272

Epitope Target: ODC

Epitope Species: human

Product Reactivity/Detection: ODC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for ODC Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin