ZW10 Antibody (3363C4a): sc-81430

ZW10 Antibody (3363C4a) is a mouse monoclonal IgG1 antibody that detects ZW10 protein of human origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). Anti-ZW10 antibody (3363C4a) is available as the non-conjugated form. ZW10 plays a crucial role in ensuring accurate chromosome segregation and proper kinetochore function during cell division, which is vital for maintaining genomic stability. As a key component of the mitotic checkpoint, ZW10 interacts with centromeres during prophase and anaphase, and with kinetochore microtubules during metaphase, effectively preventing premature exit from mitosis. ZW10 localization is dynamic throughout the cell cycle, transitioning from the cytoplasm during interphase to the kinetochore and spindle midzone during metaphase and late anaphase, respectively. Additionally, ZW10 is implicated in membrane trafficking between the Golgi apparatus and the endoplasmic reticulum (ER) through interaction with the SNARE complex. Disruption of ZW10 expression, whether through overexpression or silencing, adversely affects the ER-Golgi transport system and alters the morphology of the ER-Golgi intermediate compartment, underscoring ZW10′s essential role in inter-compartmental protein transport.

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ZW10 Antibody (3363C4a) References:

1. The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore.  |  Scaërou, F., et al. 2001. J Cell Sci. 114: 3103-14. PMID: 11590237
2. Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi.  |  Hirose, H., et al. 2004. EMBO J. 23: 1267-78. PMID: 15029241
3. ZW10 links mitotic checkpoint signaling to the structural kinetochore.  |  Kops, GJ., et al. 2005. J Cell Biol. 169: 49-60. PMID: 15824131
4. Role of the kinetochore/cell cycle checkpoint protein ZW10 in interphase cytoplasmic dynein function.  |  Varma, D., et al. 2006. J Cell Biol. 172: 655-62. PMID: 16505164
5. RINT-1 regulates the localization and entry of ZW10 to the syntaxin 18 complex.  |  Arasaki, K., et al. 2006. Mol Biol Cell. 17: 2780-8. PMID: 16571679
6. Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control.  |  Lin, YT., et al. 2006. Oncogene. 25: 6901-14. PMID: 16732327
7. Correlation of Golgi localization of ZW10 and centrosomal accumulation of dynactin.  |  Arasaki, K., et al. 2007. Biochem Biophys Res Commun. 359: 811-6. PMID: 17560939
8. ZW10 helps recruit dynactin and dynein to the kinetochore.  |  Starr, DA., et al. 1998. J Cell Biol. 142: 763-74. PMID: 9700164
9. ZW10 function in mitotic checkpoint control, dynein targeting and membrane trafficking: is dynein the unifying theme?  |  Vallee, RB., et al. 2006. Cell Cycle. 5: 2447-51. PMID: 17102640

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZW10 Antibody (3363C4a) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-ZW10 Antibody (3363C4a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZW10 Antibody (3363C4a) Specifications

Alternate Product Name: ZW10

Product Clone Name: 3363C4a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: ZW10

Epitope Species: human

Product Reactivity/Detection: ZW10

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for ZW10 Antibody (3363C4a): N/A